 |
Bromodeoxyuridine (BrdU) was added to the lukocytes enriched plasma to
achieve the final concentration of 0.1 M, and the suspension was left at
37 oC for for 1 hour. And, the cell suspension was placed on a polycarbonate
film and allowed to sediment for further 1 hour. The excess plasma was
withrawn and the cells were covered with 5 microm thick polycrbonate film
(see irradiation facility and cell preparation). The cells were irradiated
with 13.79 keV X-rays (on-peak energy that was slightly higher energy than
the K-shell binding energy (see left panel). The chromosomal effects were
compared with those irradiated with X-rays of off-peak energy (12.40keV)
To confirm possible
Auger effects (enhancement of chromosomal effects like high LET radiation
at unit dose, thymidine (1 M) was added instead of BrdU, processed in the
same way, and irradiated with on-peak energy X-rays.
The results have
nor been previously published in Journal, because the addition of thymidine
alone rather enhanced the chromosomal susceptibity, probably due to nucleotide
imbalance ??
Data not published previously, but
were collaborated with K. Kobayashi, K. Hieda, Y. Furusawa, and Y. Ejima. |